Americans with Disabilities Title III
Prohibits discrimination in “places of public accommodations", including online assets like websites. In many cases, this will mean ensuring that all video content is captioned, content is accessible via screen reader software, and content is accessibile as per WCAG 2.1.
Section 508 (Rehabilitation Act)
Specifically requires federal agencies to ensure that information and communication technology they develop, procure, maintain, or use allows employees and individuals with disabilities who are members of the public to have access to and use of information and data.